ISOLATION AND PURIFICATION OF 3-MERCAPTOPYRUVATE SULFURTRANSFERASE FROM THE GUT OF RHINOCEROS LARVA (Oryctes rhinoceros)

ISOLATION AND PURIFICATION OF 3-MERCAPTOPYRUVATE SULFURTRANSFERASE FROM THE GUT OF RHINOCEROS LARVA (Oryctes rhinoceros)

  • Format: Ms Word Document| Pages: 85 | Price: N 3,000| Chapters: 1-5
  • [btnsx id=”22434″]

3-MERCAPTOPYRUVATE SULFURTRANSFERASE

ABSTRACT
Cyanide is known to be one of the most toxic substances present in a wide variety of food materials that are consumed by animals.
One of the cyanide detoxifying enzymes is 3-mercaptopyruvate sulfurtransferase (3-MST). Indeed, recent studies have clearly shown that 3-MST is involved in the detoxification of cyanide.
Rhinoceros (Oryctes rhinoceros) larva feeds on dead, decayed and living plants, wood and palm. Plants are known to contain cyanide as a defence mechanism for intruding/pesting organisms. Thus, for rhinoceros larva to be able to live on plants, it must have possessed a cyanide-detoxifying enzyme.
3-MST, a cyanide-detoxifying enzyme was purified from Rhinoceros (Oryctes rhinoceros) larva in this work.
The 3-MST enzyme was isolated from the gut of Oryctes rhinoceros larvae and purified using Ammonium Sulphate Precipitation, Bio-Gel-P-100 Gel Filtration Chromatography and Reactive Blue-2-Agarose Affinity chromatography.
The specific activity of the enzyme was 0.22U/mg.
The presence of this enzyme could be exploited by including it in the diet of animals which would serve as a source of protein and 3-MST. Perhaps, these rhinoceros larva could be introduced on farmland with contaminated soil whereby they will process the dead roots and plants into soil thereby providing more space and manure for plants to grow healthy.

Get the Complete Project

Search
search
search engine by freefind advanced

Paper Information

Format: Ms Word Document
Pages: 46
Price: N 3,000

About admin

Check Also

SCREENING OF SENNA ALATA LEAVES FOR BIOCHEMICAL AND HYPOGLYCEMIC ACTIVITY

SCREENING OF SENNA ALATA LEAVES FOR BIOCHEMICAL AND HYPOGLYCEMIC ACTIVITY Format: Ms Word Document| Pages: 85 …

A STUDY OF THE NUTRITIVE PROFILE OF A VEGETABLE, PIPER METHYSTICUM, LOCALLY KNOWN AS AWA

A STUDY OF THE NUTRITIVE PROFILE OF A VEGETABLE, PIPER METHYSTICUM, LOCALLY KNOWN AS AWA Format: Ms …

TRACE ELEMENTS CONTENT OF NEEM LEAVES (AZADIRACHTA INDICA)

TRACE ELEMENTS CONTENT OF NEEM LEAVES (AZADIRACHTA INDICA) Format: Ms Word Document| Pages: 85 | Price: N …

THE EFFECTS OF SENNA TORA LEAVES EXTRACT ON THE BLOOD GLUCOSE LEVELS OF THE DIABETIC ALBINO RATS (A FOCUS ON DIABETES MELLITUS)

  THE EFFECTS OF SENNA TORA LEAVES EXTRACT ON THE BLOOD GLUCOSE LEVELS OF THE …

HEPATOPRODUCTIVE EFFECTS OF SENA TORA LEAVE EXTRACT ON CARBON TETRACHLORIDE INDUCED TOXICITY

HEPATOPRODUCTIVE EFFECTS OF SENA TORA LEAVE EXTRACT ON CARBON TETRACHLORIDE INDUCED TOXICITY Format: Ms Word …

EVALUATION OF ANTIOXIDANT POTENTIAL OF MONODORA MYRISTICA (AFRICAN NUTMEG)

EVALUATION OF ANTIOXIDANT POTENTIAL OF MONODORA MYRISTICA (AFRICAN NUTMEG) Format: Ms Word Document| Pages: 85 | Price: …

Leave a Reply

Your email address will not be published. Required fields are marked *